Fig. 4
IP3-induced Ca2+ flux is reduced through F1628L-, R1850Q-, and R2524C-mutant IP3R3. HEK-3KO cells, not expressing any IP3R subtype, were stably transduced with the wild-type (WT OE – overexpressing) or mutated versions of the IP3R3 receptor, subcloned, and stimulated with different concentrations of the GPCR agonist carbachol (CCH) in Ca2+ imaging buffer. Cells expressing only endogenous IP3R3 (WT) and HEK-3KO cells were used as positive and negative controls, respectively. Dose–response curves of the AUC of representative cell lines stably expressing IP3R3 with the A F1628L (n = 3), B R1850Q (n = 3), or C R2524C (n = 3) variant are shown on the left. Fold changes in IP3R3 protein expression in transduced cells normalized to endogenous IP3R3 protein expression were determined after WB (for representative blots, see Supplementary Fig. 6B) and are indicated in brackets and plotted on the right. Dotted lines indicate the reference protein expression of the WT. Values are presented as the mean + SEM
