Fig. 6
Antigen receptor downstream phosphorylation events are reduced in P2. PBMCs were stimulated with anti-CD3 and anti-CD28 antibodies or immunoglobulins for 5, 15, or 30 min. A Representative histograms illustrating Erk phosphorylation following TCR stimulation after different stimulation intervals. B Percentage of T cells with phosphorylated Erk among (un)stimulated cells. C Fold change of median fluorescence intensity in pErk+ cells compared to that in the total unstimulated cell population. D Representative histograms illustrating Erk phosphorylation following BCR stimulation after different stimulation intervals. E Percentage of B cells with phosphorylated Erk in (un)stimulated cells. F Fold change of median fluorescence intensity in pErk+ cells compared to that in the total unstimulated cell population. Number of independent experiments: A–C n = 4 with 4 different HDs. D–F n = 6 with 5 different HDs. Individuals were sampled at multiple time points. The values are presented as the mean + SEM. One-way ANOVA was performed with a multiple comparison test
