Fig. 1 | Cellular & Molecular Immunology

Fig. 1

From: Identification of common and distinct origins of human serum and breastmilk IgA1 by mass spectrometry-based clonal profiling

Fig. 1

Experimental design. A Three individual donors with matching serum and milk samples were selected for longitudinal IgA1 Fab clonal profiling. Samples were collected at three time points spaced approximately one month apart (see Supplementary Table S1 for further details). The predominant IgA1 structures in human serum (monomer, with indicated Fab and Fc) and milk (secretory dimer, with J-chain and SC), with their expected concentration ranges, are indicated in the key at the bottom. B Human serum and milk were subjected to selective IgA capture, followed by IgA1 digestion (by OgpA), and LC-MS analysis of the released intact IgA1 Fab molecules to provide clonal repertoires. Each LC-MS peak with a unique mass and retention time was annotated as a unique clone. C From these 18 samples, we compared the IgA1 Fab clonal profiles over time within and between donors and sample types. Human serum and milk IgA1 repertoires were compared for unique and shared clones between donors and biofluids

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