Fig. 3 | Cellular & Molecular Immunology

Fig. 3

From: Identification of common and distinct origins of human serum and breastmilk IgA1 by mass spectrometry-based clonal profiling

Fig. 3

Shared clone identity in serum and milk confirmed by top-down ETD-based MS/MS analyses. A clones m1 and m2 refer to the spiked-in mAb standards, and clones (A, B, and C) (annotated as [RT] [clone] [mass]) were selected as shared clones between serum and milk of Donor 1. Data for serum and milk are shown as red and blue droplets, respectively. Each square in the heatmap represents a Pearson correlation value between 0 and 1 of the raw top-down ETD MS/MS spectra of the two Fab clones. B Mirrored top-down ETD MS/MS spectra of clones (A–C) in serum (red) and milk (blue). For each of the selected clones the fragmentation spectra in serum and milk are compared, resulting in the Pearson correlation value (r) given. See Supplementary Fig. 1 for illustrative top-down ETD MS/MS data of non-paired clones, displaying correlations below 0.05

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