Fig. 7

eCIRP induces the formation of the STAT3-βPIX complex. A Binding between STAT3 and βPIX was predicted by 3D computational modeling. B RAW 264.7 cells were incubated with rmCIRP (1 µg/ml) with or without stattic (3 µM) for 24 h. Then, βPIX was pulled down by immunoprecipitation, and STAT3 levels were determined by Western blotting. Representative blots are shown. The presented data are from one experiment. The experiment was repeated twice. Data are expressed as the mean ± SD (n = 3/group). The PBS control was set to 1 for normalization. The groups were compared by one-way ANOVA and the SNK method. *p < 0.05 vs. PBS; ^#p < 0.05 vs. rmCIRP. C RAW 264.7 cells were incubated with rmCIRP (1 µg/ml) with or without stattic (3 µM) for 24 h, and then STAT3 was pulled down from the cell lysate by immunoprecipitation. The levels of free βPIX in the flow-through after STAT3 immunoprecipitation were determined by Western blotting. Representative blots are shown. The presented data are from two independent experiments. Data are expressed as the mean ± SD (n = 6/group). The PBS control was set to 1 for normalization. The groups were compared by one-way ANOVA and the SNK method. *p < 0.05 vs. PBS control; ^#p < 0.05 vs. rmCIRP. TCL total cell lysate