Table 1 Tools currently used to deplete pDCs or to inhibit their IFN production
From: The role of plasmacytoid dendritic cells (pDCs) in immunity during viral infections and beyond
Mutant mice | Genetic background | Principle/Advantage | Penetrance1 | tDCs impacted | Other cell types impacted |
|---|---|---|---|---|---|
CX3CR1-hDTR [65] | C57BL/6 | Depletion of precursors common to pDCs, tDCs and cDCs | >90% | YES | cDC2 |
CD11c-Cre; Tcf4-/fl (CKO) [66] | C57BL/6 | Loss of Tcf4 exclusively in CD11c+ cells, defect in pDC and tDC development | >90% | YES | ND2 |
Tcf4-/- [66] | 129SvEvTac | defect in pDC development | >90% | YES | tDCs, pDC-like cells, monocytes, macrophages, and subsets of B cells |
IkarosL/L [67] | C57BL/6 | Hypomorphic Ikaros mutation, defect in pDC development | 95% | ND | B cells, T cells, neutrophils |
Siglech-hDTR [68] | C57BL/6 | Bacterial artificial chromosome-based transgenic mice expressing hDTR under the control of Siglech regulatory regions | 90-95% | ND | Marginal Zone Macrophages (MZM), pDC precursors and other specialized macrophages (e.g. microglia) |
Siglech-hDTR [69] | C57BL/6 | KI mice expressing hDTR under the control of the Siglech promoter | 95% | ND | ND |
CLEC4C-hDTR/BDCA2-hDTR [70] | C57BL/6 | Transgenic mice expressing hDTR under the control of human CLEC4C/BDCA2 regulatory regions | 90-95% | NO | NO |
Antigen + (antibody clone) | Targeted host | Principle/Advantage | Penetrance | Are tDCs impacted? | Other cell types impacted? |
Mouse | Depletion of pDCs as they express high levels of Ly6C | 95% | ND | Neutrophils, monocytes, eosinophils, macrophages and activated T cells | |
Depletion of pDCs as they express high levels of Bst2 | 94% | YES | tDCs, activated monocytes and B cells | ||
SiglecH (440c Ab clone) [73] | Inhibition of IFN production by pDCs | 90% | ND | Macrophages and DC progenitors | |
BDCA2 (Litifilimab) [74] | Human and Macaques | Depletion of pDCs as they express BDCA2 | Data not shown | ND | ND |
ILT7 (VIB7734) [75] | Depletion of pDCs as they express ILT7 | 50% in human, 80% in macaque | NO | ND |
