Table 2 Mutant mice currently available and used to visualize pDCs

pDC-Tom

(Siglech^iCre;

Pacsin1^LSL-tdT) [24]

Model of intersectional genetics based on exclusive coexpression of Siglech and Pacsin1 in pDCs. Siglech^iCre remove a loxP-STOP-loxP sequence upstream of a gene encoding tdTomato (tdT) reporter located 3’ of the Pacsin1 gene. Only pDCs express tdT.

80%

NO

NO

SCRIPT (Siglech^iCre ; Ifnb1^EYFP; Pacsin1^LSL-tdT) [24]

Crossing of pDC-Tom mice with Ifnb1^EYFP mice allow to discriminating IFN-producing pDCs (tdT+ YFP + ) from nonproducing ones (tdT+ YFP-)

80%

NO

NO

ZeST (Zbtb46^GFP; Siglech^iCre; Pacsin1^LSL-tdT) [24]

Crossing of pDC-Tom mice with Zbtb46^GFP mice allows discriminating pDCs (tdT⁺ GFP^-) from tDCs and cDCs (tdT^- YFP⁺)

80%

YES

YES (cDC and DC precusors)

DPE-GFP [76]

The gene encoding GFP is under the control of the distal and proximal enhancers and promoter of mouse Cd4 gene

95%

YES

T cells

DPE-GFP x Rag1-KO [77]

Crossing DPE-GFP with Rag1-KO mice results in GFP expression exclusively in CD4⁺ myeloid cells

95%

YES

YES

Siglech-GFP [70]

The gene encoding GFP is under the control of the Siglech gene

90%

N.D.

YES (SiglecH⁺ macrophages)

pDCre-tg [78]

The gene encoding the Cre recombinase is under the control of a BAC sequence containing the Siglech gene

30%

YES

YES (DCs, DC precursors, T, B, NK cells)

Siglech^iCre [79]

The gene encoding the Cre recombinase is under the control of the endogenous Siglech gene

95%

YES

YES (DCs, DC precursors, T, B, NK cells)