Fig. 4
α4β7 integrin mediates kidney ILC2 retention. A Expression of Madcam1, Vcam1, Cdh1, and Fn1 in various clusters of kidney cells from public mouse kidney scRNA-seq data assessed using KidneyCellExplorer (http://cello.shinyapps.io/kidneycellexplorer/). Representative flow cytometry plot (B) and frequencies (C) of MAdCAM-1, VCAM-1, E-cadherin, and fibronectin expression in endothelial (CD31+), epithelial (EpCAM+), and other nonimmune cells (CD45− CD31− EpCAM−) from the naïve mouse kidney (n = 9). D Expression of key adhesion molecules in the renal intrinsic cells from the IMQ model (n = 9). E Receptor‒ligand interactions between ILC2s and renal intrinsic cell clusters through Itga4 (up) and Itgb7 (down) expressed on ILC2s, as assessed by calculating the ligand‒receptor score (L-R score) from the analysis using the SingleCellSignalR algorithm. F Graphical summary showing the predicted interactions between ILC2s and renal intrinsic cells. All results are shown as the means ± SEMs, and the statistical analysis was performed using the Mann‒Whitney U test. ns not significant; ***P < 0.001. E-cad E-cadherin, FN fibronectin, Epi epithelial cells, Endo endothelial cells, PT proximal tubule cells
