Fig. 5
Integrin α4β7 guides renal ILC2 adhesion and migration. A Experimental design for evaluating the adhesion of kidney ILC2s to prospective ligands expressed on renal tissues using live cell imaging. B Relative adhesion of ILC2s to the ligands compared to the control group (control, n = 10; MAdCAM-1, VCAM-1, and E-cadherin, n = 7; fibronectin, n = 3). C Images of ILC2s adhered to ligand-coated plates. Scale bars, 10 μm. D Trajectories of the ILC2s tracked over time on ligand-coated plates. E Velocity, accumulated distance, and Euclidian distance of the ILC2 trajectory on ligand-coated plates. F Expression of integrin α4β7 and β1 in human kidney ILCs (n = 4). G Images of human blood ILCs adhered to ligand-coated wells. Scale bars, 10 μm. All results are shown as the means ± SEMs, and the statistical analysis was performed using the Kruskal‒Wallis test. ns not significant, *P < 0.05; ***P < 0.001; and ****P < 0.0001
