Fig. 2

Monitoring of EV uptake in vivo via Cre-LoxP and single-cell sequencing. A Quantification of particles isolated from the supernatant (SN) of WT/KO Pan02 cells (mean ± SEM, n = 10). B, C s.c. tumor growth and weight of Bag6 WT/KO groups upon treatment with GW4869 (n = 3–6). D Confocal images of tumor tissues. GFP⁺ cells correspond to cre recombination events and cre-negative Bag6 KO tumors were used as negative control. E UMAP depiction of cell characterization based on cell markers. F UMAP projections of cre⁺ KO (red, left panel) and cre⁺ WT tumors (blue, left panel). Recombination events in WT (middle) and KO (right) are highlighted in green. Statistical significance: (A, C) unpaired Mann-Whitney U test; (B) or two-way ANOVA followed by Bonferroni corrections for multiple comparisons test; *P < .05; **P < 0.01; ***P < 0.001; ****P < 0.0001; n.s. (not significant); UMAP Uniform Manifold Approximation and Projection