Fig. 3

Compared with control mice, aged CD30//CD19-Cre mice have more GC B cells and more IgG1-switched cells. A FACS plots are pregated on living B220⁺ lymphocytes, as shown in Supplementary Fig. 5a. GC B cells were gated as CD95^highCD38⁻ cells, as indicated in the FACS plots. The graph summarizes the percentages of GC B cells in young and aged CD30//CD19-Cre mice (red) and control mice (blue) (N = 12–25 per group). B FACS plots depicting the gating strategy for plasmablasts/plasma cells (B220⁻CD138⁺) in young and aged CD30//CD19-Cre (red) mice and control mice (blue). The FACS plots are pregated on singlets, live cells and a large lymphocyte gate, as shown in Supplementary Fig. 5b. The percentages of PCs in different analyses are compiled in the accompanying graph (N = 11–24). C The graph compiles the percentages of isotype-switched IgG1⁺ B cells of the indicated genotypes at the indicated ages (N = 10–18 per group). IgG1-switched B cells were gated as single living CD19⁺IgD⁻ lymphocytes as indicated in Supplementary Fig. 5a and b. D The graph shows the percentages of isotype- switched IgG1 (red), IgG2b (gray) and IgG3 (blue) cells in aged CD30//CD19-Cre mice and aged control mice. A–C Data from 18 flow cytometric analyses. Two-way ANOVA with Tukey’s multiple comparisons test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001