Fig. 4

Activated CD4+ T cells express CD30-L A Graphs summarizing the percentages of effector memory T cells in the CD4⁺ and CD8⁺ subsets in young and aged CD30//CD19-Cre mice (red) and control mice (blue). Effector memory CD4⁺ and CD8⁺ T cells were gated as CD62L^lowCD44⁺ cells, as indicated in Supplementary Fig. 6a (arrow 1) (N = 16–25 per group). Data from 18 flow cytometric analyses. B SA-T cells were gated as CD4⁺CD44^highPD-1⁺ T cells. FACS plots were pregated on singlets and live cells. The plots were subsequently gated on lymphocytes, Thy1.2⁺ cells and CD4⁺ cells, as shown in Supplementary Fig. 6a (arrow 2). The surface expression of CD30-L (CD153) in the gate of SA-T cells is depicted in histograms, in which the stainings with the anti-CD153 antibody (green) and the corresponding isotype control (gray) are overlaid. The graphs compile percentages of CD4⁺CD44⁺PD-1⁺ T cells (left graph), percentages of CD30-L-expressing T cells within CD4⁺CD44⁺PD-1⁺ T cells (middle graph) and total numbers of CD153⁺CD4⁺CD44⁺PD-1⁺ T cells from CD30//CD19-Cre mice (red) and controls (CD19-Cre^+/- or CD30^stopfl/+) (blue) (N = 3–8 mice per group). C Tfh cells were gated as CD4⁺CD44⁺Bcl6⁺ cells as indicated in the FACS plot. The FACS plots were pregated in the same way as described for the SA-T cells in (B). The surface expression of CD30-L (CD153) in the gate of Tfh cells (Thy1.2⁺CD4⁺CD44⁺Bcl6⁺) is depicted in the histograms, which show an overlay of the CD153 staining and the corresponding isotype control. The graphs compile percentages of CD4⁺CD44⁺Bcl6⁺ T cells (left graph), percentages of CD30-L-expressing T cells within CD4⁺CD44⁺Bcl6⁺ T cells (middle graph) and total numbers of CD153⁺CD4⁺CD44⁺Bcl6⁺ T cells (right graph) from CD30//CD19-Cre mice (red) and controls (CD19-Cre^+/– or CD30^stopfl/+) (blue) (N = 3–7 mice per group). The ages of the mice in the different groups from (B) and (C) are indicated in the figure legends. A 2-way ANOVA with Tukey’s multiple comparisons test. B + C, ordinary one-way ANOVA with Tukey’s multiple comparisons test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001