Fig. 6

CXCR4 levels are increased in GC B cells from transgenic CD30//Cγ1-Cre mice. All analyses shown in this figure are from the spleen. FACS plots are pregated on singlets, live cells, lymphocytes and B220⁺ cells, as shown in Supplementary Fig. 5a, and on GC B cells, as shown in Fig. 5C. A The FACS plot overlay of reporter⁺ GC B cells from CD30//Cγ1-Cre mice (red) and controls (blue) illustrates the upregulation of CXCR4 in GC B cells from CD30//Cγ1-Cre mice. The graph compiles the median fluorescence intensity (MFI) of CXCR4 in GC B cells from both indicated genotypes on day 11 postimmunization (N = 9–10 per group). Rounds of immunization: 6. B FACS plots showing a representative example of the gating strategy for dark zone (DZ) (CXCR4^highCD86^low) and light zone (LZ) (CXCR4^lowCD86^high) reporter⁺ GC B cells. The graphs below summarize the percentages of DZ and LZ reporter⁺ GC B cells 11 days after immunization (N = 9–10 per group). Rounds of immunization: 6. C The histogram overlays show the CXCR4 levels in reporter⁺ GC and reporter⁺ non-GC B cells (gated as shown in Fig. 5) in comparison to those in reporter^‒ cells at the indicated time points postimmunization. The graphs below summarize the MFIs from CXCR4 in reporter⁺ GC B cells (right graph) and reporter⁺ non-GC B cells (left graph) in comparison to those in reporter^‒ cells (N = 3‒5 per group). A + B, Unpaired t test. C 2-way ANOVA with Sidak’s multiple comparisons test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001