Fig. 1
From: Critical and differential roles of eIF4A1 and eIF4A2 in B-cell development and function
eIF4A1 and eIF4A2 are required for early B cell development. A RT‒qPCR analysis of eIF4A1 and eIF4A2 mRNA expression in bone marrow B lineage cells. B, C RT‒qPCR B and immunoblot C analysis of eIF4A1 and eIF4A2 mRNA and protein expression in naïve B cells activated with various stimuli for the indicated amounts of time. D–K Flow cytometry analysis of B lineage cells in the bone marrow of Eif4a1fl/fl, Eif4a1fl/fl;Mb1Cre D–G and Eif4a2fl/fl, Eif4a2fl/fl;Mb1Cre H–K mice. D, E, F, H, I, J Representative FACS plots. G, K Bar graphs summarizing cell numbers. Each symbol represents an individual mouse. The error bars represent the standard errors of the means (SEMs). ns, not significant; *p < 0.05, **p < 0.01 and ***p < 0.001. The data shown are representative of three independent experiments. In G, K, the data represent the combination of two independent experiments
