Fig. 2
From: Critical and differential roles of eIF4A1 and eIF4A2 in B-cell development and function
eIF4A1 and eIF4A2 are indispensable for the T-cell-dependent B cell response. A, B, F, G Flow cytometry analysis of GCB (CD38-CD95+) A, F and plasma cells (B220low/-CD138+) B, G in the spleens of Eif4a1fl/fl (n = 10), Eif4a1fl/fl;CD19Cre (n = 7) A, B and Eif4a2fl/fl (n = 10), and Eif4a2fl/fl;CD19Cre (n = 6) F, G mice on day 7.5 after immunization with OVA/alum/LPS. Left, representative FACS plots; right, summary of the percentage and number of GCB and plasma cells. C, D, E, H, I, J Eif4a1fl/fl (n = 8), Eif4a1fl/fl;CD19Cre (n = 6), Eif4a2fl/fl (n = 7), and Eif4a2fl/fl;CD19Cre (n = 6) mice were immunized with NP-OVA/Alum. Serum concentrations of NP-specific IgM C, H and IgG1 D, E, I, J antibodies at the indicated time points were determined by ELISA. Each symbol represents an individual mouse. The error bars represent the standard errors of the means (SEMs). ns, not significant; *p < 0.05, **p < 0.01 and ***p < 0.001. The data shown are representative of three independent experiments
