Fig. 5
From: Critical and differential roles of eIF4A1 and eIF4A2 in B-cell development and function
eIF4A2 is required for 40S ribosome biogenesis. A Outline of polysome profiling experiments. B, C Polysome profiling graphs of Eif4a1fl/fl, Eif4a1fl/fl;CD19Cre B, Eif4a2fl/fl, Eif4a2fl/fl;CD19Cre C B cells stimulated with LPS for 24 h. D, E Immunoblot analysis of eIF4A1, eIF4A2, and ribosomal proteins in the indicated sucrose gradient fractions of B-cell lysates. Red underlines indicate the absence of RPS6 protein in the 40S fractions of Eif4a2-deficient B cells E. F, G Heatmap of RPS F and RPL G proteins in WT (C57BL/6 J), Eif4a1fl/fl;CD19Cre and Eif4a2fl/fl;CD19Cre B cells stimulated with LPS for the indicated durations. Two-way ANOVA was performed. The asterisks on the right side of each panel indicate the differences between WT and Eif4a1- or Eif4a2-deficient B cells at 24 hours after LPS stimulation F, G. *p < 0.05, **p < 0.01 and ***p < 0.001. The data shown are representative of three independent experiments. Each column represents an individual biological sample
