Fig. 6
From: Critical and differential roles of eIF4A1 and eIF4A2 in B-cell development and function
eIF4A2 is required for 18S rRNA maturation. A Immunoblot analysis of eIF4A2 and ribosomal proteins in Eif4a2fl/fl and Eif4a2fl/fl;CD19Cre B cells stimulated with LPS for the indicated amounts of time. B, C Heatmap of the mRNA levels of the RPS B and RPL C genes determined by RNA-seq analysis of Eif4a2fl/fl and Eif4a2fl/fl;CD19Cre B cells stimulated with LPS for the indicated amounts of time. D The 18S/28S rRNA ratio was quantified via the Agilent 5400 Fragment Analyzer System to measure the amounts of 18S and 28S rRNA in total RNA from Eif4a2fl/fl and Eif4a2fl/fl;CD19Cre B cells stimulated with LPS for the indicated amounts of time. E Eif4a2fl/fl and Eif4a2fl/fl;CD19Cre B cells were stimulated with LPS for 12 hours, treated with MG132 or DMSO for the indicated amounts of time, and analyzed by immunoblotting. F Scheme of 18S rRNA maturation. G, H Northern blot analysis of total RNA from B cells stimulated with LPS for 24 hours using ITS-29 G, U3, U6, U14, and U22 probes H. The error bars represent the standard errors of the means (SEMs). ns, not significant; *p < 0.05, **p < 0.01 and ***p < 0.001. The data shown are representative of three independent experiments
