Fig. 2

IL-33-induced EoE is characterized by eosinophilia, epithelial hyperplasia, and increased number of esophageal ILCs. A Schematic representation of acute and chronic EoE. B H&E-stained sections of control (d0), acute (d7), and chronic (d28) EoE mouse esophagi. Scale bars = 50 µm. (Epi: epithelium, LP: lamina propria, Mus: muscle). Quantification of epithelial thickness (C) and basal cell layer thickness (D) in EoE model mice. E Immunohistochemistry images of major basic protein 1 (MBP1)⁺ cells in EoE mouse esophagi. Flow cytometry plots showing eosinophils (F) and frequencies of esophageal eosinophils (G) in the acute and chronic EoE mouse models. Comparison of CCR3⁺ eosinophils in acute and chronic EoE. Histogram (H) and CCR3 gMFI from eosinophils (I). J Comparison of CCR3-associated chemokine gene expression in whole esophageal tissues with EoE. Analysis of CCR3-associated chemokine gene expression (K) and epithelium-associated gene expression (L) in IL-33-overexpressing mouse esophageal public RNA sequencing data. Flow cytometry plots (M) and frequencies (N) of esophageal-resident ILCs. O Schematic representation of acute EoE in eosinophil-deficient dblGATA1 mice. P Representative H&E-stained sections of control and acute EoE from dblGATA1 mice. Quantification of epithelial thickness (Q) and basal cell layer thickness (R) in dblGATA1 EoE model mice. All scale bars = 50 µm. The data were pooled from at least 2‒3 independent experiments and are presented as the means ± SEMs. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, ns, not significant