Fig. 5

Pim2 expression in macrophages is involved in the development of inflammatory arthritis. A Schematic of CIA induction in Pim2^fl/fl-Lyz2^Cre mice. B CIA scores and hind paw thicknesses of Pim2^fl/fl and Pim2^fl/fl-Lyz2^Cre mice (n = 6). C Macroscopic images, micro-CT, H&E, and safranin O staining of the ankles of Pim2^fl/fl and Pim2^fl/fl-Lyz2^Cre mice (n = 6). D The expression of TNF-α, IL-1β, IL-6 and IL-10 in Pim2^fl/fl and Pim2^fl/fl-Lyz2^Cre mice after CIA induction, as detected by IHC. E The expression of iNOS and Arg-1 in synovial macrophages from Pim2^fl/fl and Pim2^fl/fl-Lyz2^Cre mice, as detected by IF staining (F4/80, green; iNOS, red; Arg-1, red). F, G ECARs and OCRs of BMDMs isolated from Pim2^fl/fl and Pim2^fl/fl-Lyz2^Cre mice after M1 induction (n = 4). H, I MFIs of MHC-II and CD206 in F4/80+ BMDMs isolated from Pim2^fl/fl and Pim2^fl/fl-Lyz2^Cre mice after M1/M2 induction, as detected by flow cytometry (n = 6). J‒M mRNA and protein expression levels of inflammatory factors in BMDMs isolated from Pim2^fl/fl and Pim2^fl/fl-Lyz2^Cre mice after M1/M2 induction, as determined by RT‒qPCR and Western blotting (n = 6)