Fig. 8
NM@NP-Bex inhibits macrophage M1 polarization and alleviates arthritis in CIA model mice in a targeted and safe manner. A, B The MFIs of HLA-DR and CD206 were detected in CD68+ hMDMs subjected to different treatments after M1/M2 induction, as determined by flow cytometry (n = 3). C Arthritis severity was determined via the CIA score (n = 6). D Macroscopic images, micro-CT, H&E, and safranin O staining of ankles from mice subjected to different treatments (n = 6). E The expression of TNF-α, IL-1β, IL-6 and IL-10 in mice subjected to different treatments after CIA induction was detected by IHC (n = 6). F The expression of iNOS and Arg-1 in synovial macrophages from mice subjected to different treatments was detected by immunofluorescence staining (F4/80, green; iNOS, red; Arg-1, red) (n = 6)
