Fig. 3

Lung γδ⁺ T cells are the major source of IL-17A in the lungs of PPE-induced emphysema mice. A The frequency and quantification of Siglec-F⁺ neutrophils in the lungs of WT and Rag1^KO mice 4 days after PPE instillation (n = 5 per group). B The frequency and absolute number of IL-17A-expressing cells among T-cell subsets in the lungs of WT mice were analyzed on day 2 after PPE instillation (n = 4 per group). C Experimental protocol for anti-Vγ2 TCR administration in the PPE-induced emphysema model. The frequency of Siglec-F⁺ neutrophils in the lungs of PPE-induced emphysema mice was assessed on day 4 after depletion of γδ⁺ T cells (n = 5 per group). D Absolute number of Siglec-F⁺ neutrophils in the lungs of PPE-induced emphysema mice on day 4 after depletion of γδ⁺ T cells (n = 5 per group). E Lung tissue histology and the MLI on day 4 in PPE-instilled WT mice pretreated with either anti-IgG or anti-Vγ2 antibodies. Scale bar = 200 μm (n = 5 per group). F The frequency of γδ⁺ T cells in the lungs of PPE-induced emphysema mice two days after the administration of cytokine-blocking antibodies (n = 4 per group). Unpaired two-tailed Student’s t test with Welch’s correction A left-C, and E, unpaired one-way ANOVA with Dunnett’s T3 post hoc test F, and unpaired two-way ANOVA with Tukey’s post hoc test A right, D were used to measure significance. *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant; error bars indicate the means ± SDs