Fig. 8 | Cellular & Molecular Immunology

Fig. 8

From: γδ+ T-cell-derived IL-17A stimulates airway epithelial/stromal cells to secrete G-CSF, promoting lung-specific pathogenic Siglec-F+ neutrophil development in PPE-induced emphysema

Fig. 8

Depletion of Siglec-F+ neutrophils or inhibition of G-CSFR signaling alleviated emphysema symptoms. WT mice were treated i.t. with anti-Ly6G antibody A or anti-Siglec-F antibody B before instillation with PPE. Lung tissue histology after H&E staining and MLI assessment were conducted 4 days after PPE instillation. Scale bar = 200 μm (n = 4 per group in each experiment). PPE-instilled mice were treated i.t. with AG490 (JAK2 inhibitor) C, HJC0152 (STAT3 inhibitor) D, rapamycin (mTOR inhibitor) E, or Adezmapimod (p38 inhibitor) F. Lung tissue histology after H&E staining and MLI assessment were conducted 4 days after PPE instillation. Scale bar = 200 μm (n = 4 per group). Unpaired two-tailed Student’s t test with Welch’s correction was used to measure significance. *P < 0.05, **P < 0.01, ***P < 0.001; the error bars indicate the means ± SDs

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