Fig. 5

Identification of the stroke-associated endothelial cell subset that mediates Tsa cell infiltration. A UMAP of the 15 major cell populations identified in the peri-infarct cortex in the stroke group and the corresponding regions in the sham group. Left: Integration of data from the sham and stroke groups. Top middle: the sham group. Bottom middle: The stroke group. Right: Stacked bar graphs showing the proportions of different cells in the sham and stroke groups. B Cell type annotation was performed on the basis of the expression of well-established marker genes. The color of the dots indicates the expression level. The dot size represents the proportion of cells expressing the indicated markers. C Feature plot of Ccl19 expression in all cells. D Schematic of endothelial cells, microglia, astrocytes, and neurons isolated from the brains of tMCAO stroke model mice. E Relative expression of Ccl19 in the sorted cells as determined by qPCR in the sham and tMCAO mice. n = 3 samples for each group; each sample was pooled from 3 animals. F UMAP of the 10 cell populations identified by endothelial cell clustering. Left: Integration of data from the sham and stroke groups. Top right: the sham group. Bottom right: The stroke group. G Dot plot showing the expression of Ccl19 in endothelial cell subsets. H Bar graph showing the percentages of the various endothelial cell subtypes identified in (F) in both the sham and stroke groups. Dot plot showing the expression of Ccl19 in the endo-7 subset in both the sham and stroke groups. I Relative expression of CCL19 in the brains of non-stroke controls and AIS patients, as determined by qPCR. n = 6/group. J CCL19 expression in the brains of non-stroke controls and AIS patients was measured via western blotting. n = 6/group. The data are presented as the means ± SEMs. *P < 0.05; **P < 0.01; ***P < 0.001