Fig. 6

CCL19-CCR7 coordinates endothelial cell-Tsa cell cross-talk. A Schematic diagram of Ccl19 lentiviral construction, AAV injection, tMCAO, flow cytometry, infarction examination, ELISA, neurological deficit assessment, and behavioral studies. B Endothelial cells were sorted from the brain tissues of C57BL/6J mice after AAV-TIE2-siRNA-Ccl19 injection. The relative expression of Ccl19 in the sorted cells was determined by qPCR. n = 6/group. C Flow cytometry analysis of the proportion of Tsa cells in the peri-infarct area of stroke mice with downregulated Ccl19. D The levels of the inflammatory factors TNF-α and IFN-γ in the peri-infarct area of tMCAO + AAV-TIE2-siRNA-NC– and tMCAO+AAV-TIE2-siRNA-Ccl19–injected mice were measured via ELISA 24 h after stroke. n = 6/group. E Representative TTC-stained sections from tMCAO+AAV-TIE2-siRNA-NC– and tMCAO+AAV-TIE2-siRNA-Ccl19–injected mice at 24 h after stroke, followed by analysis of infarct volume. n = 10/group. F Neurological deficit scores of AAV-TIE2-siRNA-NC– and AAV-TIE2-siRNA-Ccl19–injected mice 24 h  after stroke. n = 10/group. Effects of Ccl19 knockdown on behavioral recovery at different time points after stroke, as measured by the grid walking test (G), cylinder test (H), and adhesive removal test (I). n = 10/group. The data are presented as the means ± SEMs. **P < 0.01; ***P < 0.001; ^#P < 0.05; ^##P < 0.01; ^###P < 0.001