Fig. 2
Altered IL-9 signaling during obesity and improved metabolic and inflammatory profiles in response to rIL-9 treatment: Mice were fed a normal chow diet (NCD) or high-fat diet (HFD) for 12 weeks, and A frequencies of IL-9 + CD4+ T cells in the SVF of adipose tissue were analyzed; B gene expression of IL-9R in the adipose tissue of NCD-fed and HFD-fed mice; C MFI of IL-9R in the adipose tissue macrophages of NCD-fed and HFD-fed mice; D insulin tolerance test (ITT) of HFD-fed mice treated i.p. with PBS or 2 µg/kg rIL-9 thrice weekly; E the area under the curve (AUC) of the ITT; F weight gain; G Adipose tissue eosinophils; H RELMα+ macrophages and I ILC2 frequencies in HFD-fed mice treated i.p. with PBS or 2 µg/kg recombinant IL-9 thrice weekly. All experiments were repeated twice, and representative data are shown. *p < 0.05, compared with HFD-fed PBS by two-way ANOVA. For D and F, two-way ANOVA was performed, and for the remaining two-tailed, unpaired t tests were performed
