Fig. 2

scRNA-seq reveals macrophage heterogeneity in sAH. A Single-cell RNA sequencing analysis was performed on liver cells from 5 healthy controls, 3 AC patients, and 5 sAH patients, as well as peripheral white blood cells (WBCs) from 3 AC patients and 4 sAH patients. t-SNE plots showing the clustering of all the macrophage populations. B t-SNE plots showing macrophage clusters separated by patient group. C Heatmap illustrating the key signature genes of each macrophage subtype. D Heatmap displaying the expression of phagocytosis-related genes across macrophage subtypes. (E) Heatmap showing the expression of cytokine genes among all the macrophage subtypes