Fig. 4

Bulk RNA-seq revealed differentially expressed genes in the livers of healthy controls and AC and sAH patients. A Bulk RNA-seq data of liver tissues from healthy controls and AC and sAH patients were analyzed. The PCA plot displays different gene expression patterns in healthy controls and AC and sAH patients. B Volcano plot showing differentially expressed genes in the livers of sAH patients and healthy controls. The x-axis indicates the log2-fold change (sAH patients vs. healthy controls), and the y-axis indicates the –log10 p value. The blue dots represent downregulated genes (log2[fold change] < −1, p < 0.05), and the red dots represent upregulated genes (log2[fold change] > 1, p < 0.05). C Heatmap showing the expression of neutrophil degranulation-related genes (upper panel), neutrophil apoptotic process-related genes (middle panel), and apoptotic cell clearance-related genes (lower panel) in the livers of healthy controls and AC and sAH patients