Fig. 7
Comparison of liver injury and neutrophil infiltration in WT and Apoe KO mice in the EtOH-feeding model. A WT mice and Apoe KO mice were subjected to the chronic-plus-single binge model. Serum ALT and AST levels were measured. Liver tissues were subjected to HE staining, IHC staining of neutrophils and macrophages, and Sirius Red staining. The quantification of neutrophils, macrophages, and Sirius Red is shown. B Liver tissues from WT and Apoe KO mice were subjected to bulk RNA-seq analysis. The PCA plot shows different gene expression patterns between pair-fed and EtOH-fed Apoe KO and WT mice. C Volcano plot displaying differentially expressed genes in the livers of EtOH-fed Apoe KO and WT mice. The x-axis represents the log2-fold change (Apoe KO vs. WT), and the y-axis represents the –log10 p value. The blue dots represent downregulated genes (log2[fold change] < −1, p < 0.05), and the red dots represent upregulated genes (log2[fold change] > 1, p < 0.05). D Reactome pathway analysis of differentially expressed genes in the livers of EtOH-fed Apoe KO and WT mice. E Heatmap showing the expression of neutrophil degranulation-related genes in the livers of Apoe KO and WT mice. The values are presented as the means ± SEMs (A, B). *P < 0.05, **P < 0.01, ***P < 0.001, as determined by a 2-tailed Student’s t test for comparing two groups
