Fig. 2

The absence of B cells impairs CD8⁺ T-cell priming after viral infection. A Proliferation of adoptively intravenously transferred OVA-specific transgenic OTI CD45.1⁺CD8⁺ T cells at 3 days after the induction of MCMV-SIINFEKL infection. The bar graphs show the frequencies (left) and absolute numbers (right) of proliferating OTI CD45.1⁺CD8⁺ T cells in each group (n = 4). B Flow cytometry dot plot showing CD62L^-CD44⁺ effector OTI CD45.1⁺CD8⁺ T cells at 3 days after the induction of MCMV-SIINFEKL infection. The bar graphs indicate the frequencies (left) and absolute numbers (right) of CD62L^-CD44⁺ effector OTI CD45.1⁺CD8⁺ T cells in each group (n = 4). C Immunofluorescence images of B220 and MCMV-EGFP in the spleens of WT mice, 48 hours after MCMV-EGFP infection, with uninfected WT mice serving as controls (scale bar, 50 µm; n = 4). D Schematic diagram outlining the coculture of OVA-specific transgenic OTI CD8⁺ T cells with B cells isolated from MCMV-SIINFEKL-infected WT mice. E Flow cytometry histograms showing the proliferation of OVA-specific transgenic OTI CD8⁺ T cells cocultured with B cells isolated from uninfected or MCMV-SIINFEKL-infected WT mice (n = 5). The data are presented as the means ± SEMs and are representative of two independent experiments. Statistical analysis: one-way ANOVA, Fig. 2A, 2B