Fig. 4: Immunofluorescent stains in vivo

a, c Wound healing studies of SD rat models injected with DFO-hydrogels to observe antibacterial effects in vivo. Representative images showing immunofluorescent stains with Staphylococcus aureus antibodies (green) and DAPI counterstaining (nucleus, blue). The summarized data showed the differences in the staining intensities of Staphylococcus aureus among the different groups. Scale bars = 100 μm. b, d Histological changes of wound healing in the DFO-hydrogel-injected wounds. Immunofluorescence staining of vascular endothelial cells treated with DFO-hydrogels. The cells were stained with CD31, and the nuclei were counterstained with DAPI. The summarized data showed the differences of the staining intensities of CD31 among the different groups. Scale bars = 100 μm