Fig. 3: Matrix stiffening reverted the activation of astrocytes.
From: Matrix stiffness changes affect astrocyte phenotype in an in vitro injury model
a Schematic depiction of matrix stiffening. b The cell spreading area significantly decreased when the matrix was stiffened after 7 days of culture. c Matrix stiffening influenced the shape of astrocytes. d, e Immunofluorescence analysis indicated that the activation of astrocytes decreased when the matrix was stiffened after 7 days (red, IL-1β; green, GFAP; blue, nucleus). Scale bar, 50 μm. f Relative protein levels of GFAP determined by Western blotting in astrocytes after 7 days and 14 days of culture.
