Fig. 4: YAP inhibition enhanced the responses of astrocytes to matrix stiffness.
From: Matrix stiffness changes affect astrocyte phenotype in an in vitro injury model
a, b Immunofluorescence analysis suggested that increasing matrix stiffness reduced the expression of the YAP protein and upregulated the expression of the GFAP protein (red, YAP; green, GFAP; blue, nucleus). Scale bar, 50 μm. c Quantification of the ratio of nuclear YAP to cytoplasmic YAP in astrocytes. d Quantification of the normalized total YAP levels. e Immunofluorescence analysis indicated that astrocyte activation increased when astrocytes in a stiff matrix were transfected with siRNAs targeting YAP (blue, nucleus; green, GFAP). Scale bar, 50 μm. f Quantification of GFAP levels when astrocytes in a stiff matrix were transfected with siRNAs targeting YAP.
