Fig. 2: The multifunctional efficient and protective cationic dendrimer G5-AHP.
From: Injectable “nano-micron” combined gene-hydrogel microspheres for local treatment of osteoarthritis

a The number of each amino acid conjugated to the dendrimer was characterized by 1H NMR. b The particle size and zeta potential of G5-AHP at N/P ratios of 0.5, 1, 2, 4, and 8. c The microstructure of G5-AHP/miR-140 was observed under TEM. d The G5-AHP/miR-140 nanoparticle binding capacity was investigated by agarose gel electrophoresis experiments. e Protective ability of G5-AHP/miR-140 polyplexes from RNase A. f The transfection efficacy of G5-AHP/miR-140 polyplexes was observed by LSCM. g Statistical analyses of integrated fluorescence density. n = 3. *P < 0.05. h, i The positive cell rate and mean fluorescence intensity in cells treated with G5-AHP/miR-140 polyplexes by flow cytometry. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001.