Fig. 5: 3D-MMP design for tumor-vasculature coculture model and fluorescence imaging of tumor spheroid on capillary bed. | NPG Asia Materials

Fig. 5: 3D-MMP design for tumor-vasculature coculture model and fluorescence imaging of tumor spheroid on capillary bed.

From: 3D micromesh-based hybrid bioprinting: multidimensional liquid patterning for 3D microtissue engineering

Fig. 5

A Detailed design and patterning procedure of 3D-MMP. Cancer zone (inner mesh) consists of 400 μm gap, vascular zone (outer mesh) consists of 1000 μm gap. U-87 MG spheroid was placed in cancer zone by direct injection, HUVECs and LFs are patterned in vascular zone by dipping. B, C Confocal microscope images of both experimental group (HUVECs and LFs exist in the vascular zone) and control group (Only fibrin gel exists in the vascular zone) in Day 5. D Section view of vascularized tumor spheroid. Spheroid maintains spherical form on micro vascular bed. Scale bar = 200 μm (B), Scale bar = 400 μm (C).

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