Fig. 5: In vivo delivery of cells that secrete melanin using B16-loaded and Mela-loaded S-cryoMNs.

A Timeline of the delivery of B16 melanoma cells and human primary melanocytes to the immunodeficient model mice. The dermabrasion method was compared with the S-cryoMNs delivery methods. B Melanin expression and (C) quantitative analysis of the transplanted B16 cells using dermabrasion, blank or B16-loaded S-cryoMNs 1 and 3 days after application. Melanin was stained with Masson-Fontana stain and is indicated by the red arrow. At each time point, three independent samples were analyzed. D Melanin expression and (E) quantitative analysis of the transplanted melanocytes obtained via dermabrasion, blank or Mela-loaded S-cryoMNs 1 and 3 days after application. Melanin was stained with Masson-Fontana stain and is indicated by the red arrow. The scab is indicated using a green arrow. At each time point, three independent samples were analyzed. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001; ns no significant difference. n = 3.