Fig. 1: Inhibition of proliferation and colony-forming ability of osteosarcoma cells by Pt NCs.

A The line graph illustrates the variations in cell viability of MG-63 and U2-OS cells when exposed to specific concentration gradients (ranging from 0 to 0.8 μg/mL) of Pt NCs and cisplatin. B The EdU immunofluorescence results demonstrate the disparity in the proliferative activity of MG-63 and U2-OS cells when treated with distinct concentrations of Pt NCs, thereby indicating the inhibitory effect of Pt NCs on cell proliferation. C The statistical plot illustrates the EdU expression in MG-63 and U2-OS cells, where the presence of EdU fluorescence indicates cells in a state of proliferation. D The clonogenesis assay results and statistical plot revealed variations in the quantity and cellular dimensions of clones generated by MG-63 and U2-OS cells when exposed to specific concentrations of Pt NCs, thus indicating the suppressive impact of Pt NCs on the clonogenic potential of cells.