Fig. 3: Promotion of apoptosis in osteosarcoma cells by Pt NCs. | NPG Asia Materials

Fig. 3: Promotion of apoptosis in osteosarcoma cells by Pt NCs.

From: Novel platinum nanoclusters (Pt NCs) induce mitochondrial apoptosis and damaging autophagy for the treatment of osteosarcoma—from the perspective of P53 mutation status in different cell lines

Fig. 3

A Flow cytometry results in MG-63 and U2-OS cells revealed a significant disparity in the proportion of cells undergoing apoptosis following treatment with specific concentrations of Pt NCs, thereby demonstrating the inhibitory impact of Pt NCs on apoptosis in osteosarcoma cells. Cells residing within the Q2 and Q4 quadrants denoted cells in an apoptotic state, while those within the Q1 quadrant indicated cells in a deceased state. Cells within the Q3 quadrant represented viable cells. B, C The protein expression levels and statistical plot of P53 protein and apoptosis signature protein were examined in MG-63 and U2-OS cells treated with specific concentrations of Pt NCs. This analysis confirmed the disparity in P53 expression between MG-63 and U2-OS cells, while also demonstrating the impact of Pt NCs on apoptosis. BAX and Caspase-3 were the positive apoptosis signature proteins, while Bcl-2 were the negative apoptosis signature proteins.

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