Fig. 6: Effects of Pt NCs on autophagy in MG-63 (P53−). | NPG Asia Materials

Fig. 6: Effects of Pt NCs on autophagy in MG-63 (P53−).

From: Novel platinum nanoclusters (Pt NCs) induce mitochondrial apoptosis and damaging autophagy for the treatment of osteosarcoma—from the perspective of P53 mutation status in different cell lines

Fig. 6

A Autophagic flux detection of mRFP-GFP-LC3. Red and yellow fluorescence represent autolysosomes and autophagosomes, respectively. An increase in the number of these two fluorophores per cell indicates increased autophagic flux. B Western blot of autophagy positive signature protein (LC3B) in different groups, representing autophagy. C Changes in apoptosis flow cytometry in different groups. Cells residing within the Q2 and Q4 quadrants denoted cells in an apoptotic state. B, C 3-MA is an autophagy inhibitor. Divided into the following four groups: the blank control group (NC), the group with only 3-MA added, the group with only Pt NCs added, and the group added with 3-MA and Pt NCs.

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