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Bioactive peptide-bearing polylactic acid fibers as a model of the brain tumor-stimulating microenvironment

Polymer Journal volume 55, pages 273–281 (2023)Cite this article

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Abstract

Malignant glioblastoma multiforme (GBM), with an aggressive growth pattern and unpredictable tumor location within the brain, causes a deadly disease with an extremely poor prognosis. Laminin, a typical matrix in the basement membranes of the brain vasculature and GBM-remodeled extracellular matrix (ECM), exhibits a tumor-promoting effect. Here, a poly(L–lactic acid) (PLLA) fiber substrate was modified with an IKVAV peptide derived from laminin to construct an in vitro model for investigating the effect of the ECM on GBM in white matter tracts. A heterofunctional compound composed of oligo(D–lactic acid) (ODLA) and the IKVAV peptide was synthesized and linked with a hydrophilic diethylene glycol linker (ODLA–diEG–IKVAV) to form PLLA/ODLA–diEG–IKVAV aligned fibers. The hydrophilicity and IKVAV surface density were improved by heating, enhancing human astrocytoma (U-251MG) cell adhesion. The bioactive peptide scaffold constructed in this study is a promising platform that can be used to investigate the effects of the ECM on brain tumors.

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Acknowledgements

This work was supported by JSPS KAKENHI (grant number 16H03186).

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Authors and Affiliations

  1. Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, 6-1 Kishibe-Shimmachi, Suita, Osaka, 564-8565, Japan

    Wan-Ying Huang, Akihisa Otaka & Tetsuji Yamaoka

  2. Department of Frontier Fiber Technology and Science, University of Fukui, Fukui, 910-8507, Japan

    Satoshi Fujita

Authors
  1. Wan-Ying Huang
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  2. Akihisa Otaka
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  3. Satoshi Fujita
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  4. Tetsuji Yamaoka
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Contributions

HW-Y: Writing—original draft, conceptualization, methodology, investigation, visualization, and validation. AO: Conceptualization, methodology, investigation, project administration, validation, writing—review, and editing. SF: Methodology, investigation, validation, writing—review, and editing. TY: Conceptualization, methodology, resources, supervision, writing—review, and editing.

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Correspondence to Tetsuji Yamaoka.

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Huang, WY., Otaka, A., Fujita, S. et al. Bioactive peptide-bearing polylactic acid fibers as a model of the brain tumor-stimulating microenvironment. Polym J 55, 273–281 (2023). https://doi.org/10.1038/s41428-022-00743-8

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  • DOI: https://doi.org/10.1038/s41428-022-00743-8

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