Fig. 1

Rho expression in the nucleus of yeast cells induces a growth defect. a Ten-fold serial dilutions of yeast cells transformed with the empty vector or the indicated plasmids expressing Rho-NLS or Rho-ΔNLS were spotted on agar plates in the presence or absence of 5 μg/ml of Doxy and grown at 25 °C for 3 days. b Kirby-Bauer disk diffusion test. Agar plates with or without 5 μg/ml Doxy were seeded with a lawn of S. cerevisiae bearing the Rho-NLS expression plasmid. Paper disks were dispensed on the agar surface and spotted with 7 μl of a solution at 100 mg/ml of Bicyclomycin (BCM, bottom disk) or 7 μl of water (top disk) for the control. Pictures were taken after 3 days incubation at 25 °C. c Western blot using anti-Rho antibodies showing Rho expression upon induction by omission of Doxy in the growth medium in the absence or presence of 100 μg/ml of BCM. The lower band corresponds to a yeast protein cross-reacting non-specifically with the anti-Rho antibodies