Fig. 3 | The Journal of Antibiotics

Fig. 3

From: Recombinant yeast and human cells as screening tools to search for antibacterial agents targeting the transcription termination factor Rho

Fig. 3

Rho expression in EcR 293 cell lines induces a cytostatic effect. a Western blot showing the conditional expression of Rho-NLS and Rho-ΔNLS by the recombinant EcR 293 cell lines. Pona stands for the inducer Ponasterone which was added to the cell culture medium at 5 µM. The upper band corresponds to a protein cross-reacting non-specifically with the anti-Rho antibodies. b Western blot showing the expression of Rho-NLS as a function of time. c Photograph showing the growth over 7 days of the recombinant EcR 293 cell lines expressing conditionally Rho-NLS or Rho-ΔNLS. The addition of BCM to the relevant culture at 50 μg/ml final concentration was done 12 h after cells seeding

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