Abstract
Secondary metabolites of microorganisms have proven to be an excellent source of drugs. We isolated a new antibiotic, named pestiocandin (1), from a culture broth of a filamentous fungus, Pestalotiopsis humus FKI-7473, using a multidrug-sensitive budding yeast, S. cerevisiae 12geneΔ0HSR-iERG6. The structure of 1 was elucidated by various NMR studies. All geometric isomerisms of 1 were shown to be the E-form and two pyranose units of 1 were found to be glucose and galactose types. Compound 1 showed weak growth inhibition against Gram-positive and Gram-negative bacteria, yeasts and a filamentous fungus. It displayed more potent growth inhibition against multidrug-sensitive yeasts than wild-type yeasts.
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Acknowledgements
This research was supported by The Public Foundation of Elizabeth Arnold–Fuji, Japan. We thank Dr Kenichiro Nagai and Ms Noriko Sato, School of Pharmacy, Kitasato University, for measurement of MS and NMR spectra.
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Sakai, K., Suga, T., Iwatsuki, M. et al. Pestiocandin, a new papulacandin class antibiotic isolated from Pestalotiopsis humus. J Antibiot 71, 1031–1035 (2018). https://doi.org/10.1038/s41429-018-0102-7
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DOI: https://doi.org/10.1038/s41429-018-0102-7
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