Abstract
A new antifungal compound YO-001A was found from the culture broth of Streptomyces sp. YO15-A001, which was isolated from a soil sample collected in Toyama Prefecture. YO-001A was identified through morphological changes-based screening of the rice blast fungus, Pyricularia oryzae (P. oryzae). YO-001A is a new 26-membered macrolide of the oligomycin family, which exhibits potent antifungal activity against P. oryzae with an IC50 of 0.012 µM by disrupting mitochondrial respiration via inhibition of the FOF1-ATPase activity.
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Acknowledgements
We thank Mr S. Hasegawa, Ms N. Ogita, and Mr T. Abukawa for their help with the construction of morphological database of P. oryzae; Ms A. Okano, Dr T. Miyazawa, Mr Y. Furuyama, Ms A. Ishiyama, and Ms M. Osada for isolating microorganisms from soil sample; Dr S. Takahashi and Dr A. Khalid for performing the 16S rRNA sequencing; Dr T. Nogawa for measurement of HR-ESI-MS; Dr J. Otaka and Ms M. Tanaka for evaluating the biological activity; Dr Julius Adam V. Lopez and Mr Fauze Bin Mahmud for paper editing. This work was supported in part by the JSPS KAKENHI Grant numbers JP17H06400, JP15H02445, JP18H03945, and JP17K01970 and grants from the Project of the NARO Bio‐oriented Technology Research Advancement Institution (Research program on development of innovative technology, 28011A).
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Yamamoto, K., Futamura, Y., Uson-Lopez, R.A. et al. YO-001A, a new antifungal agent produced by Streptomyces sp. YO15-A001. J Antibiot 72, 986–990 (2019). https://doi.org/10.1038/s41429-019-0239-z
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DOI: https://doi.org/10.1038/s41429-019-0239-z
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