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Study on the mechanisms of the cross-resistance to TET, PIP, and GEN in Staphylococcus aureus mediated by the Rhizoma Coptidis extracts

The Journal of Antibiotics volume 74, pages 330–336 (2021)Cite this article

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Abstract

The purpose of this study was focused on the mechanisms of the cross-resistance to tetracycline (TET), piperacillin Sodium (PIP), and gentamicin (GEN) in Staphylococcus aureus (SA) mediated by Rhizoma Coptidis extracts (RCE). The selected strains were exposed continuously to RCE at the sublethal concentrations for 12 days, respectively. The susceptibility change of the drug-exposed strains was determined by analysis of the minimum inhibitory concentration. The 16S rDNA sequencing method was used to identify the RCE-exposed strain. Then the expression of resistant genes in the selected isolates was analyzed by transcriptome sequencing. The results indicated that RCE could trigger the preferential cross-resistance to TET, PIP, and GEN in SA. The correlative resistant genes to the three kinds of antibiotics were upregulated in the RCE-exposed strain, and the mRNA levels of the resistant genes determined by RT-qPCR were consistent with those from the transcriptome analysis. It was suggested from these results that the antibacterial Traditional Chinese Medicines might be a significant factor of causing the bacterial antibiotic-resistance.

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Acknowledgements

We are grateful for Professor Xian Zhang for kindly providing the clinical isolates.

Funding

This study was supported by Natural Science Foundation of Guangxi Autonomous Region (NO. 2014GXNSFAA118176), Development and Research Center for China-ASEAN Traditional Medicine and Nanning City Science and Technology Plan [NO. 20131062].

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Authors and Affiliations

  1. Institute of Pharmacy, Guangxi University of Chinese Medicine, Nanning, Guangxi, PR China

    Sugui Lan, Zhirong Li, Aiqiu Su, Yanhong Peng, Yanke Liao, Xuemei Liu & Qiang Tan

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  1. Sugui Lan
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  2. Zhirong Li
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  3. Aiqiu Su
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Correspondence to Qiang Tan.

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Lan, S., Li, Z., Su, A. et al. Study on the mechanisms of the cross-resistance to TET, PIP, and GEN in Staphylococcus aureus mediated by the Rhizoma Coptidis extracts. J Antibiot 74, 330–336 (2021). https://doi.org/10.1038/s41429-021-00407-4

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