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Streptomyces tabacisoli sp. nov, isolated from the rhizosphere soil of Nicotiana tabacum

The Journal of Antibiotics volume 78, pages 229–234 (2025)Cite this article

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Abstract

Strain HUAS MG91T, a novel actinobacterium, was isolated from the rhizosphere soil of Nicotiana tabacum collected from Changde City, China. This strain could form short, rod-shaped spores with a smooth surface and Rectiflexibles spore chains on Gause’s synthetic No.1 medium. Comparison of 16S rRNA gene sequences showed that strain HUAS MG91T exhibited the highest similarity to Streptomyces kunmingensis NBRC 14463 T (98.95%). Phylogenetic analysis based on 16S rRNA gene and genome sequences indicated that strain HUAS MG91T was closely related to S. kunmingensis NBRC 14463 T. However, the ANIm and dDDH values between them were 87.25% and 28.60%, respectively, lower than the 96.7% ANIm threshold and 70.0% cutoff point used as guideline values for Streptomyces species delineation. On the basis of phenotypic and genotypic differentiation from tested strains, strain HUAS MG91T is proposed to represent a novel species of the genus Streptomyces, named Streptomyces tabacisoli sp. nov. The type strain is HUAS MG91T (=MCCC 1K09288T = JCM 37027 T).

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Acknowledgements

All authors would like to thank MCCC (Marine Culture Collection of China, MCCC) and China Center of Industrial Culture Collection (CICC, China) for providing us with excellent technical assistance.

Funding

This work was supported by the National Natural Science Foundation of China (62472168).

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Author notes

  1. These authors contributed equally: Kaiqin Li, Hao Zhang

Authors and Affiliations

  1. School of Computer Science and Engineering, Hunan University of Science and Technology, Xiangtan, China

    Kaiqin Li & Wei Liang

  2. School of Life and Health Sciences, Hunan University of Science and Technology, Xiangtan, China

    Hao Zhang, Ying Qian & Jian Gao

  3. Institute of Synthetic Biology Industry, Hunan University of Arts and Science, Changde, Hunan Province, China

    Ping Mo & Aihua Deng

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  1. Kaiqin Li
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  7. Wei Liang
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Contributions

KL, AD, JG, and WL conceived the idea of the study and purchased type strains. KL, HZ, YQ, PM, and AD conducted an experiment, prepared figures and tables. KL and HZ wrote the main manuscript text. JG and WL corrected and reviewed the paper.

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Correspondence to Wei Liang.

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Li, K., Zhang, H., Qian, Y. et al. Streptomyces tabacisoli sp. nov, isolated from the rhizosphere soil of Nicotiana tabacum. J Antibiot 78, 229–234 (2025). https://doi.org/10.1038/s41429-024-00802-7

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