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Streptomyces maremycinicus sp. nov. and its secondary metabolite-biosynthetic gene clusters

The Journal of Antibiotics volume 78, pages 560–568 (2025)Cite this article

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Abstract

Streptomyces strain TP-A0890T, isolated from a soil sample, is a producer of FR-900452 and A-74863a. The taxonomic status was clarified by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Streptomyces coriariae, with similarity of 99.7%. Strain TP-A0890T comprised ll-diaminopimelic acid, glutamic acid, glycine and alanine in its peptidoglycan. The predominant menaquinones were MK-9(H8) and MK-9(H6), and major fatty acids were anteiso-C17:0, anteiso-C15:0, iso-C16:0 and iso-C17:0. The chemotaxonomic features matched those described for the genus Streptomyces. The genome size and G + C content were 8.72 Mb and 71.5%, respectively. The results of digital DNA-DNA hybridization along with differences in phenotypic characteristics between the strains suggested strain TP-A0890T to assign to a novel species, for which Streptomyces maremycinicus sp. nov. is proposed; the type strain is TP-A0890T ( = NBRC 110468T). We also show that Streptomyces strain B9173, a producer of FR-900452 and maremycins that was isolated from coastal sediment in Chile, belonged to S. maremycinicus. Twenty-two to 23 secondary metabolite-biosynthetic gene clusters (smBGCs) were present in the genomes of S. maremycinicus strains. Seventeen of them were conserved in the genome of S. coriariae CMB-FBT but the others were not.

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Acknowledgements

We are grateful to Ms. Satomi Saitou for assistance of taxonomic experiments. We also thank Ms. Yuko Kitahashi and Ms. Aya Uohara for finishing the genome sequences and registering the genome sequences in the DDBJ, respectively. We thank Dr. Aharon Oren for reviewing specific epithets and the etymologies.

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Authors and Affiliations

  1. Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, 292-0818, Japan

    Hisayuki Komaki & Tomohiko Tamura

  2. Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Toyama, 939-0398, Japan

    Yasuhiro Igarashi

Authors
  1. Hisayuki Komaki
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  2. Yasuhiro Igarashi
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Correspondence to Hisayuki Komaki.

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Komaki, H., Igarashi, Y. & Tamura, T. Streptomyces maremycinicus sp. nov. and its secondary metabolite-biosynthetic gene clusters. J Antibiot 78, 560–568 (2025). https://doi.org/10.1038/s41429-025-00844-5

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