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Streptomyces cyclosori sp. nov., a novel actinobacterium from the rhizosphere soil of Cyclosorus parasiticus (L.) Farw

The Journal of Antibiotics volume 78, pages 666–673 (2025)Cite this article

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Abstract

A novel actinobacterium strain cg36T, from the rhizosphere of Cyclosorus parasiticus, was subjected to a polyphasic taxonomic study. 16S rRNA gene sequence analysis showed that strain cg36T shared the highest 16S rRNA gene sequence similarity to Streptomyces lavendofoliae NBRC 12882T. Phylogenetic analysis of 16S rRNA gene sequence indicated that strain cg36T formed a distinct branch within the genus Streptomyces. Phylogenetic analysis of five housekeeping gene and whole genome sequences showed that strain cg36T was closely related to Streptomyces crystallinus JCM 5067T and Streptomyces noboritoensis JCM 4557T. But, overall genome related index (OGRI) and multilocus sequence analysis (MLSA) confirmed that strain cg36T was clearly different from them. A comparison of differential features among strain cg36T, S. crystallinus CGMCC 4.1600T and S. noboritoensis CGMCC 4.1457T provided, at least to some extent, some useful information for strain cg36T as an independent species. In addition, OGRI values and MLSA evolutionary distance between strain cg36T and other strains, which had 16S rRNA gene sequence similarity of >98.65% to strain cg36T, further demonstrated that strain cg36T was a new species. Whole-cell hydrolysates of strain cg36T contained ll-diaminopimelic acid and whole-cell sugars contain glucose. The predominant cellular fatty acids (>10%) were anteiso-C15:0, iso-C15:0 and C16:0. The DNA G + C content of the genome sequence, consisting of 9,022,416 bp, was 72.5%. All these data indicated that strain cg36T represents a novel Streptomyces species, for which the name Streptomyces cyclosori sp. nov. is proposed. The type strain is strain cg36T (=MCCC 1K09286T = JCM 37520T).

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Acknowledgements

We would like to express appreciation to Prof. Aharon Oren (Department of Plant and Environmental Sciences and the Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem) for his assistance with the etymology check.

Funding

This work was supported by the Hunan Provincial Natural Science Foundation of China and Xiangtan Science and Technology Bureau (2022JJ50125).

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Authors and Affiliations

  1. School of Life and Health Sciences, Hunan University of Science and Technology, Xiangtan, Hunan, China

    Rongjian Gao, Yazi Chen, Yan Xiao & Jian Gao

  2. Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Xiangtan, China

    Jian Gao

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RJG and YZC conducted experiments, prepared figures and tables. RJG and YX purchased type strains. RJG and YZC wrote the main manuscript text. JG corrected and reviewed the paper.

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Correspondence to Jian Gao.

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Gao, R., Chen, Y., Xiao, Y. et al. Streptomyces cyclosori sp. nov., a novel actinobacterium from the rhizosphere soil of Cyclosorus parasiticus (L.) Farw. J Antibiot 78, 666–673 (2025). https://doi.org/10.1038/s41429-025-00857-0

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