Fig. 5
Effect of ACSL5 isoforms on cell viability. a Growth index of cells transfected with control plasmid (p-V5), nonspliced (pNSpl-V5) and spliced (pSpl-V5) constructs. Statistical significance is indicated with asterisks: *P < 0.05; **P < 0.005; ***P < 0.0005. b Basal level of ACSL5 RNA in nontransfected cells. One LCL is included to show the endogenous expression of Spl and NSpl isoforms, as a positive control. These data were obtained by real time qPCR and the amplified samples were run in a polyacrylamide gel electrophoresis. UBE2D2 expression was used as a reference. c Basal level of ACSL5 protein expression performed by western blots of nontransfected cell lines
