Fig. 3: Knockout of Gdf6 impacts migration of murine IMCD3 cells, an effect partially reversed by expression of wild-type not mutant GDF6. | European Journal of Human Genetics

Fig. 3: Knockout of Gdf6 impacts migration of murine IMCD3 cells, an effect partially reversed by expression of wild-type not mutant GDF6.

From: Rare heterozygous GDF6 variants in patients with renal anomalies

Fig. 3

a Relative to mIMCD3 cells, migration of Gdf6−/− mIMCD3 cells (clones 32 and 34) was significantly decreased compared with Gdf6+/+ mIMCD3 cell clone 2. b The effect of Gdf6 knockout in mIMCD3 cell clone 32 expressing empty vector (vector control) relative to mIMCD3 cells was partially rescued by stable expression of human wild-type (WT) GDF6 in mIMCD3 cell clone 32. Conversely, relative cell migration of Gdf6−/− mIMCD3 cell clone 32 stably expressing GDF6 c.112G>C or c.746C>A variants was significantly reduced compared with Gdf6−/− mIMCD3 cell clone 32 stably expressing wild-type GDF6. All results are mean ± SD from three independent experiments. Scale bar: 150 µm. N.s. not significant; **p < 0.01; ***p < 0.001.

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