Table 2 Overview of the number of genes associated with only SAT, only SAT/TAT, with both SAT and SAT/TAT, and their total sum, in the analysis of 30,917 genes in the study population from the EPIC-Potsdam substudy (n=160).

From: Identification of novel genes whose expression in adipose tissue affects body fat mass and distribution: an RNA-Seq and Mendelian Randomization study

Description

SAT only

SAT/TAT only

SAT and SAT/TAT

Total

Associated genesa

400

184

41

625

Associated autosomal genesa

392

177

38

607

Associated autosomal genes that are knownb

69

38

13

120

Associated autosomal genes that are novelb

323

139

25

487

Associated autosomal genes which encode known proteinc

338

158

35

531

  1. aafter Bonferroni-correction for multiple testing of the 30,917 genes.
  2. bOverlap with genes that have been associated with obesity and body fat distribution in previous studies on the molecular or genetic level. These lists have extracted on July 25, 2020 from the (i) NCBI gene database (https://www.ncbi.nlm.nih.gov/gene) by searching and extracting all associated with “obesity” in humans, and (ii) GWAS Catalog (https://www.ebi.ac.uk/gwas/), where all SNVs (associations) with a p value <10−5 for all relevant reported traits and child traits were extracted when searching for EFO (Experimental Factor Ontology) traits “obesity”, “fat body mass”, “body mass index”, “body composition measurement“, “body fat distribution”, “BMI-adjusted waist-hip ratio”, “visceral:subcutaneous adipose tissue ratio” and “visceral:total adipose tissue ratio”, and restricting the results to main overall effects (i.e. ignoring interaction effects, subgroup analyses, and proxy traits such as protein levels of obesity as traits), and restricting to body fat distribution traits of the trunk/abdomen (i.e. ignoring e.g. leg fat distribution). (iii) Further, the AstraZeneca PheWAS Portal was accessed at https://azphewas.com on June 25, 2022 and genes were extracted that were associated with BMI, waist circumference, whole body fat mass, abdominal SAT mass or VAT mass in gene-level or variant level association tests. (i) yielded 1962 genes, (ii) SNVs in 2509 genes, and (iii) 23 genes and SNPs in 562 genes, which yields in total 4460 known candidate genes for obesity and body composition.
  3. cWhether each gene encodes a known protein was checked using the UniProt annotation from https://biodbnet-abcc.ncifcrf.gov/db/db2db.php, queried on July 25, 2020.
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